Canada - Bothwell Cheese Inc. is recalling shredded cheeses for possible Listeria monocytogenes contamination

Food Poisoning Bulletin

In Canada, Bothwell Cheese Recalled for Listeria

December 20, 2015 by Carla Gillespie

 

Bothwell CheeseInc. is recalling shredded cheeses for possible Listeria monocytogenes contamination. Consumers who have purchased these cheeses should not eat them as Listeria can cause serious illness.

The recalled cheeses were sold in Quebec, Manitoba and Saskatchewan. They include: White Cheddar Shred, Old Cheddar Shred, Mild Shred and Old Shred in 10kg packages with Lot # 151030. And Mozzarella Blend Shredded Cheese in 340g packages with lot number Lot # 160626 Best Before JUN 26 16 and UPC 0 58898 34082 1.

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Syngenta mixed GMOs into US crops to force the world to accept untested biotechnology, lawsuit claims

NaturalNews

Monday, December 14, 2015 by: J. D. Heyes


(NaturalNews) Agri- and biotech giant Syngenta is being sued by hundreds of farmers in at least 20 states for shocking business practices, including using the American people as GMO guinea pigs.
According to Arkansas Business, one of the lawsuits against the Swiss seed manufacturer, which has been filed on behalf of two Newport-area farms, alleges that Syngenta "has engaged in a criminal conspiracy to contaminate the U.S. corn crop to force China (and) other nations that buy U.S. corn and U.S. farmers to accept" GMO corn.

The publication further reported in online editions:

The suit, field by the Emerson Poynter law firm, which has offices in Little Rock and Houston, alleges that Syngenta violated the Racketeer Influenced & Corrupt Organizations Act, or RICO, which is usually used to fight organized crime.

The firm filed the class-action suit in January on behalf of Eagle Lake Farms and Kenny Falwell, both operating farms in the Newport area. The suit was filed in U.S. District Court for the Eastern District of Arkansas. At least eight other suits have also been filed against the seed maker, the news report said.

In all, there are hundreds of pending suits against Syngenta that have been filed by American farmers since last fall. The suits claim that Syngenta caused financial losses of between $1 billion and $2.9 billion to U.S. corn farmers after selling them GMO corn that China had yet to approve for use. China is a major (and growing) importer of American corn and maize-related byproducts.

Drop in value

 

In particular, the suits identified the Agrisure Viptera seed, also known as MIR 162. The suits say the seeds have been genetically altered to resist corn pests including earworms and cutworms. The seed was approved by the U.S. Department of Agriculture in 2010.

In November 2013, China began banning shipments of U.S. corn after its scientists detected the GMO trait, leading to a dramatic decrease in the price and value of U.S. corn. The suits allege that even farmers who did not use the GMO corn suffered economic losses as well.

Farmers in 20 states have filed suit. Together, they represent 86 percent of all corn planted in the United States last year, plaintiffs' lawyers said.

Arkansas Business further reported:

China went on to approve Viptera in December, but plaintiffs' lawyers say the development has little, if any, effect on their case. Scott Powell of Hare Wynn Newell & Newton of Birmingham, Alabama, is one of those lawyers.

China, with its rapidly expanding middle class, has "a voracious appetite for corn," Powell said, and when it stopped buying U.S. corn, it found other vendors, like Brazil. And once a country finds a substitute vendor for a product, it rarely switches back.

It's not just farmers lining up to sue Syngenta for its shady practice. Other agri-business giants are suing as well.

'We don't mess with China'

 

For example ADM, one of the world's largest processors of corn, filed suit against the Swiss company in November. "Syngenta chose to sell a corn seed product with traits that were not approved in all major export markets, without undertaking reasonable stewardship practices to prevent the resulting crop from commingling with or otherwise tainting the rest of the U.S. corn supply," an ADM spokeswoman told Arkansas Business.

For it's part, Syngenta says the suits are baseless, saying it "believes that the lawsuits are without merit and strongly upholds the right of growers to have access to approved new technologies that can increase both their productivity and their profitability," as reported by AgWeb.com.

The company added that it "commercialized the trait in full compliance with regulatory and legal requirements," "obtained import approval from major corn importing countries," and "has been fully transparent in commercializing the trait over the last four years."

U.S. farmers take China's business seriously.

"We don't mess with China," Deb Volnek, a Nebraska farmer involved in the suit against Syngenta, told Reuters. "When China buys something, the markets go up. When they don't, the markets go down."


Sources:

http://www.arkansasbusiness.com
http://www.agweb.com
http://www.reuters.com

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Hawaii Recalls Korean Raw Oysters After Reports of Norovirus Illnesses

Transmission electron micrograph of Norwalk virus. The white bar = 50 nm

EPA  ...Wikipedia.org
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Food Safety News

By News Desk | December 12, 2015

Raw oysters imported from Korea and sold in bulk to distributors and food establishments in Hawaii are being recalled.

 

The Hawaii State Department of Health (DOH) said the individually quick frozen (IQF) packaged under the Dai One Food Company label, with shellfish harvest dates of Feb. 10 -13, 2015, with shellfish tags on all shellfish cases are subject to the recall.

The department has already conducted product tracebacks and embargoed all of the suspect product on Nov. 24 at various local shellfish distributors and restaurants, said Peter Oshiro.

“Although this product is not sold directly to the public, a recall has been issued as an additional safeguard to further notify anyone who may possess the product that it is unsafe and should be destroyed,” he said.

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DuPont and Dow Chemical—announce plans to merge into a $130 billion giant, taking us one giant step closer to a total monopoly on our food suppy

Published on

Friday, December 11, 2015

by

Common Dreams

The DuPont and Dow Chemical Merger: Bad Deal for People and the Planet

Groups are calling on the Department of Justice to reject the deal to protect food supply

by

Sarah Lazare, staff writer

 

 

 

"Just a handful of large chemical companies including Dow and DuPont already control most of the seed supply used to grow crops like corn and soybeans, as well as the herbicides that genetically engineered seeds are designed to be grown with," said Wenonah Hauter of Food & Water Watch. (Photo: Desmanthus4food/Wikimedia/cc)

Watchdog groups are sounding the alarm after two of the oldest and largest corporations in the United States—DuPont and Dow Chemical—announced Friday plans to merge into a $130 billion giant, thereby establishing the world's biggest seed and pesticide conglomerate.

The new behemoth, named DowDuPont, would then be split into "three independent, publicly traded companies through tax-free spin-offs," according to a joint corporate statement marking one of the the largest deals of 2015.

These companies would focus on agriculture, material science, and "technology and innovation-driven Specialty Products company," the statement continues. Together, they would form the second-largest chemical company world-wide.

The merger, if it goes through, is expected to slash numerous jobs.

And it would expand the influence of two Big Ag players, with the combined venture retaining control over "17 percent of global pesticide sales and about 40 percent of America’s corn-seed and soybean markets," according to the calculations of Washington Post analysts.

Rights groups warn that this large share would be very bad for people and the planet—and called on the Department of Justice to block the merger.

"The Department of Justice needs to block this merger to prevent the further corporate control of the basic building blocks of the food supply."

—Wenonah Hauter, Food & Water Watch

"Just a handful of large chemical companies including Dow and DuPont already control most of the seed supply used to grow crops like corn and soybeans, as well as the herbicides that genetically engineered seeds are designed to be grown with," said Wenonah Hauter, executive director of advocacy organization Food & Water Watch, in a statement released Friday.

"Any merger that consolidates this market into fewer hands will give farmers fewer choices and put them at even more economic disadvantage," Hauter continued. "And it will make it harder for agriculture to get off the GMO-chemical treadmill that just keeps increasing in speed. The Department of Justice needs to block this merger to prevent the further corporate control of the basic building blocks of the food supply."

According to the New York Times, "Despite the eventual breakup, the deal would undergo rigorous antitrust scrutiny for all three companies, particularly the agricultural chemicals company."
Diana Moss, president of the American Antitrust Institute, confirmed Friday that "any merger on the agricultural inputs side of DuPont and Dow will get antitrust scrutiny."

Nonetheless, watchdog groups warn that the merger announcement is a bad sign, in an industry that has already undergone dramatic consolidation.

"Some of the markets for biotech and seeds are highly concentrated, which has been driven by Monsanto having made so many acquisitions in the past. If you put a new merger in the this mix, it's going to raise concerns about leaving only two or maybe three firms," Moss explained. "Farmers could be squeezed even more and consumers could pay higher prices."

Robert Reich, University of California at Berkeley professor and former Secretary of Labor, took to social media to warn that the merger would result in greater political power for the corporation, as well as "higher prices for you for food and a variety of other products."

"Crop prices continue to drop, so the only way these giant companies can increase earnings is by increasing their market power to raise prices (Monsanto is also on the prowl to buy a Big Ag or chemical company)," Reich continued. "That means more of your paycheck will be going to them, directly or indirectly."

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This work is licensed under a Creative Commons Attribution-Share Alike 3.0 License

A pesticide that was present in milk in the early 1980s may be linked to signs of Parkinson's disease that are showing up in people today, according to a new study.

Pesticide In Milk May Be Linked To Parkinson's Disease

The pesticide is commonly used in Hawaii.

• Agata Blaszczak Boxe LiveScience.com

12/11/2015 11:00 am ET

MIB Pictures via Getty Images

 

By: Agata Blaszczak Boxe
Published: 12/11/2015 10:03 AM EST on LiveScience

A pesticide that was present in milk in the early 1980s may be linked to signs of Parkinson's disease that are showing up in people today, according to a new study.

The researchers looked at Japanese-American men in Hawaii, where the pesticide was frequently used, and found that those who drank more than two cups of milk daily at the start of the study had 40 percent fewer brain cells in an area of the brain called the substantia nigra 30 years later, on average, compared with similar men who drank less than two cups of milk per day.

The loss of brain cells in this area of the brain may be an early sign of Parkinson's disease, and can start decades before any visible symptoms of the disease occur, the researchers said. The study included men whose average age was 54 at the start of the study.

The link between the higher consumption of milk and the loss of cells in this brain area was not found among the men in the study who had ever smoked cigarettes, the researchers noted. Previous research has shown that people who smoke cigarettes may have a lower risk of developing Parkinson's disease, the researchers said. [10 Things You Didn't Know About the Brain]

The researchers also found that residues of the pesticide, called heptachlor epoxide, were present in the brains of 90 percent of the men who drank the most milk, compared with 63 percent of those who did not drink any milk.

High levels of the pesticide were found in the milk supply in the early 1980s in Hawaii, where the chemical was used for insect control in the pineapple industry. The commercial sale of the pesticide in agriculture was banned in the United States by 1988, according to the U.S. Environmental Protection Agency. However, a related compound can still be used today for controlling fire ants in electrical grid machinery, according to the Agency for Toxic Substances & Disease Registry.

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Study Linking GMOs and Tumors Vindicated Yet Again...MSM Stays Silent

     

corbettreport

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Published on Dec 6, 2015

SHOW NOTES: https://www.corbettreport.com/?p=17182 If you follow the mainstream media, you'll probably only know the Seralini 2012 rat feeding study as "that GMO cancer study that got retracted." You probably won't know that the paper has since been republished and Seralini himself has won two court victories defending his work. Join me for today's Thought For The Day as I break down the GMO truth that Monsanto doesn't want you to know.

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Seralini’s team wins defamation and forgery court cases on GMO and pesticide research

• 

On 25 November 2015, the High Court of Paris indicted Marc Fellous, former chairman of France’s Biomolecular Engineering Commission, for “forgery” and “the use of forgery”, in a libel trial that he lost to Prof Gilles-Eric Séralini. The Biomolecular Engineering Commission has authorised many GM crops for consumption.

 The details of the case have not yet been publicly released but a source close to the case told GMWatch that Fellous had used or copied the signature of a scientist without his agreement to argue that Séralini and his co-researchers were wrong in their reassessment of Monsanto studies.

 The Séralini team’s re-assessment reported finding signs of toxicity in the raw data from Monsanto’s own rat feeding studies with GM maize.

The sentence against Fellous has not yet been passed and is expected in June 2016.

 Defamation case

The latest ruling marks a second court victory for Séralini’s team.

 In September 2012, an article written by Jean-Claude Jaillette in Marianne magazine said that “researchers around the world” had voiced “harsh words” about the research of Séralini and his team on the toxic effects of a GMO and Roundup over a long term period – research that was supported by the independent organisation CRIIGEN. The journalist wrote of a “scientific fraud in which the methodology served to reinforce pre-determined results”.

Séralini, his team, and CRIIGEN challenged this allegation in a defamation lawsuit. They were assisted by the notaries Bernard Dartevelle and Cindy Gay.

On 6 November 2015, after a criminal investigation lasting three years, the 17th Criminal Chamber of the High Court of Paris passed sentence. Marianne magazine and its journalist were fined for public defamation of a public official and public defamation of the researchers and of CRIIGEN, which is chaired by Dr Joel Spiroux de Vendômois.

Read More Here

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Double standards used in evaluating GMO safety studies

An example of an inconclusive study that has not been retracted from FCT is the Monsanto study on the same GM maize variety that Séralini tested.

Scientific studies should be evaluated according to standards that are consistently and transparently applied to all studies, regardless of whether their findings are reassuring, surprising, or unpopular. Yet it appears that the Séralini study has been singled out for retraction under a criterion of “inconclusiveness” that has not been applied to other papers.

An example of an inconclusive study that has not been retracted from FCT is the Monsanto study on the same GM maize variety that Séralini tested (Hammond et al, 2004).[1] This study used the same strain of rat and analyzed blood and urine samples from the same number of animals as Séralini used in total.

Crucially, however, the Monsanto study was only 90 days in duration. Thus it was terminated a month before the more obvious pathologies began to appear in the Séralini study. Nevertheless, the data in the Monsanto study show statistically significant differences in multiple organ functions between the GM and non-GM feeding groups, which the Monsanto authors dismissed as not “biologically meaningful”, without proper scientific justification.

As a result, and of great importance from a public health perspective, this GM maize was passed by regulators as safe to consume on a life-long basis despite the fact that the Monsanto study was only 90 days in duration and contained scientifically questionable claims of statistically significant findings being termed as not “biologically meaningful”.

Read More Here

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19. Agribusiness Giants Attempt to Silence and Discredit Scientists Whose Research Reveals Herbicides’ Health Threats

October 1, 2014
 

Independent journalists, including E. Ann Clark, James Corbett, Rachel Aviv, and Democracy Now!, document how Big Agriculture giants Monsanto and Syngenta have attempted to silence the findings and destroy the reputations of scientists whose research shows that the companies’ herbicides pose serious threats to human health.

In September 2012, Dr. Gilles-Éric Séralini published research findings in the peer-reviewed Journal of Food and Chemical Toxicology. These findings showed the toxic impact of Monsanto’s herbicide and genetically modified corn—including adverse health effects on rats. However, after publication, the journal made the unprecedented decision to retract the study.

 Journal editor Dr. A. Wallace Hayes admitted that none of the established criteria for retracting a study applied to the Séralini paper. However, as Clark and Corbett reported, a new connection between the journal and Monsanto might account for the retraction, as well as another retraction of a similar study from Brazil that demonstrated the toxic effects on mice of an insecticide that forms the basis of the Bt GMO crops. After these papers were published, the Journal of Food and Chemical Toxicology created a new position: the associate editor for biotechnology. The journal then selected Richard E. Goodman, from the University of Nebraska, to fill the position and preside over such retractions. As it turns out, Goodman worked in regulatory sciences for Monsanto from 1997 to 2004.

  Read More Here

 

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Genetic Fallacy: How Monsanto Silences Scientific Dissent

corbettreport

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Published on Dec 3, 2013

SUPPORT BOILINGFROGSPOST.COM: http://ur1.ca/g527g TRANSCRIPT AND SOURCES: http://www.corbettreport.com/?p=8325 That a former Monsanto scientist should find himself in charge of a specially-created post at the very journal that published two landmark studies questioning the safety of that company's products should surprise no one who is aware of the Monsanto revolving door. This door is responsible for literally dozens of Monsanto officials, lobbyists and consultants finding themselves in positions of authority in the government bodies that are supposedly there to regulate the company and its actions. Find out more about Monsanto's ability to suppress scientific dissent in this week's edition of the BoilingFrogsPost.com Eyeopener report.

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Research

Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize

Gilles-Eric Séralini^1*, Emilie Clair¹, Robin Mesnage¹, Steeve Gress¹, Nicolas Defarge¹, Manuela Malatesta², Didier Hennequin³ and Joël Spiroux de Vendômois¹

• * Corresponding author: Gilles-Eric Séralini criigen@criigen.info

Author Affiliations

¹ Institute of Biology, EA 2608 and CRIIGEN and Risk Pole, MRSH-CNRS, Esplanade de la Paix, University of Caen, Caen 14032, Cedex, France ² Department of Neurological, Neuropsychological, Morphological and Motor Sciences, University of Verona, Verona 37134, Italy ³ Risk Pole, MRSH-CNRS, Esplanade de la Paix, University of Caen, Caen 14032, Cedex, France

For all author emails, please log on.

Environmental Sciences Europe 2014, 26:14 doi:10.1186/s12302-014-0014-5 See related Commentary: http://www.enveurope.com/content/26/1/13 The electronic version of this article is the complete one and can be found online at: http://www.enveurope.com/content/26/1/14

Received:	22 March 2014
Accepted:	16 May 2014
Published:	24 June 2014

© 2014 Séralini et al.; licensee Springer This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The health effects of a Roundup-tolerant NK603 genetically modified (GM) maize (from 11% in the diet), cultivated with or without Roundup application and Roundup alone (from 0.1 ppb of the full pesticide containing glyphosate and adjuvants) in drinking water, were evaluated for 2 years in rats. This study constitutes a follow-up investigation of a 90-day feeding study conducted by Monsanto in order to obtain commercial release of this GMO, employing the same rat strain and analyzing biochemical parameters on the same number of animals per group as our investigation. Our research represents the first chronic study on these substances, in which all observations including tumors are reported chronologically. Thus, it was not designed as a carcinogenicity study. We report the major findings with 34 organs observed and 56 parameters analyzed at 11 time points for most organs.

Results

Biochemical analyses confirmed very significant chronic kidney deficiencies, for all treatments and both sexes; 76% of the altered parameters were kidney-related. In treated males, liver congestions and necrosis were 2.5 to 5.5 times higher. Marked and severe nephropathies were also generally 1.3 to 2.3 times greater. In females, all treatment groups showed a two- to threefold increase in mortality, and deaths were earlier. This difference was also evident in three male groups fed with GM maize. All results were hormone- and sex-dependent, and the pathological profiles were comparable. Females developed large mammary tumors more frequently and before controls; the pituitary was the second most disabled organ; the sex hormonal balance was modified by consumption of GM maize and Roundup treatments. Males presented up to four times more large palpable tumors starting 600 days earlier than in the control group, in which only one tumor was noted. These results may be explained by not only the non-linear endocrine-disrupting effects of Roundup but also by the overexpression of the EPSPS transgene or other mutational effects in the GM maize and their metabolic consequences.

Conclusion

Our findings imply that long-term (2 year) feeding trials need to be conducted to thoroughly evaluate the safety of GM foods and pesticides in their full commercial formulations.

Keywords:

Genetically modified; GMO; Roundup; NK603; Rat; Glyphosate-based herbicides; Endocrine disruption

Research

Empirical natural and social sciences produce knowledge (in German: Wissenschaften schaffen Wissen) which should describe and explain past and present phenomena and estimate their future development. To this end quantitative methods are used. Progress in science needs controversial debates aiming at the best methods as basis for objective, reliable and valid results approximating what could be the truth. Such methodological competition is the energy needed for scientific progress. In this sense, ESEU aims to enable rational discussions dealing with the article from G.-E. Séralini et al. (Food Chem. Toxicol. 2012, 50:4221–4231) by re-publishing it. By doing so, any kind of appraisal of the paper’s content should not be connoted. The only aim is to enable scientific transparency and, based on this, a discussion which does not hide but aims to focus methodological controversies. -Winfried Schröder, Editor of the Thematic Series “Implications for GMO-cultivation and monitoring” in Environmental Sciences Europe.

Background

There is an ongoing international debate as to the necessary length of mammalian toxicity studies, including metabolic analyses, in relation to the consumption of genetically modified (GM) plants [1]. Currently, no regulatory authority requires mandatory chronic animal feeding studies to be performed for edible genetically modified organisms (GMOs), or even short-term studies with blood analyses for the full commercial formulations of pesticides as sold and used, but only for the declared active principle alone. However, several 90-day rat feeding trials have been conducted by the agricultural biotechnology industry. These investigations mostly concern GM soy and maize that are engineered either to be herbicide-tolerant (to Roundup (R) in 80% of cases), or to produce a modified Bt toxin insecticide, or both. As a result, these GM crops contain new pesticide residues for which new maximum residue levels (MRL) have been established in some countries. Though the petitioners conclude in general that no major physiological changes is attributable to the consumption of the GMO in subchronic toxicity studies [2-5], significant disturbances have been found and may be interpreted differently [6,7]. A detailed analysis of the data in the subchronic toxicity studies [2-5] has revealed statistically significant alterations in kidney and liver function that may constitute signs of the early onset of chronic toxicity. This may be explained at least in part by pesticide residues in the GM feed [6,7]. Indeed, it has been demonstrated that R concentrations in the range of 10³ times below the MRL can induce endocrine disturbances in human cells [8] and toxic effects thereafter [9]. This may explain toxic effects seen in experiments in rats in vivo[10] as well as in farm animals [11]. After several months of consumption of an R-tolerant soy, the liver and pancreas of mice were affected, as highlighted by disturbances in sub-nuclear structure [12-14]. Furthermore, this toxic effect was reproduced by the application of R herbicide directly to hepatocytes in culture [15]. More recently, long-term and multi-generational animal feeding trials have been performed, with some possibly providing evidence of safety, while others conclude on the necessity of further investigation because of metabolic modifications [16]. However, in contrast with the study we report here, none of these previous investigations have included a detailed follow-up of the animals, including multiple (up to 11) blood and urine sampling over 2 years, and none has investigated either the GM NK603 R-tolerant maize or Roundup. Furthermore, evaluation of long-term toxicity of herbicides is generally performed on mammalian physiology employing only their active principle, rather than the complete formulations as used in agriculture. This was the case for glyphosate (G) [17], the declared active chemical constituent of R. It is important to note that G is only able to efficiently penetrate target plant organisms with the help of adjuvants present in the various commercially used R formulations [18]. Even if G has shown to interact directly with the active site of aromatase at high levels [19], at low contaminating levels, adjuvants may be better candidates than G to explain the toxicity or endocrine disruptive side effects of R on human cells [8,20] and also in vivo for acute toxicity [21]. In this regard, it is noteworthy that the far greater toxicity of full agricultural formulations compared to declared supposed active principles alone has recently been demonstrated also for six other major pesticides tested in vitro[22]. When G residues are found in tap water, food, or feed, they arise from the total herbicide formulation although little data is available as to the levels of the R adjuvants in either the environment or food chain. Indeed, adjuvants are rarely monitored in the environment, but some widely used adjuvants (surfactants) such as nonylphenol ethoxylates, another ethoxylated surfactant like POEA present in R, are widely found in rivers in England and are linked with disruption of wildlife sexual reproduction [23]. Adjuvants are found in groundwater [24]. The half-life of POEA (21 to 42 days) is even longer than for G (7 to 14 days) in aquatic environments [25]. As a result, the necessity of studying the potential toxic effects of total chemical mixtures rather than single components has been strongly emphasized [26-28]. On this basis, the regular measurement of only G or other supposed active ingredients of pesticides in the environment constitute at best markers of full formulation residues. Thus, in the study of health effects, exposure to the diluted whole formulation may be more representative of environmental pollution than exposure to G alone. With a view to address this lack of information, we performed a 2-year detailed rat feeding study. Our study was designed as a chronic toxicity study and as a direct follow-up to a previous investigation on the same NK603 GM maize conducted by the developer company, Monsanto [3]. A detailed critical analysis of the raw data of this subchronic 90-day rat feeding study revealed statistically significant differences in multiple organ function parameters, especially pertaining to the liver and kidneys, between the GM and non-GM maize-fed group [3,7]. However, Monsanto's authors dismissed the findings as not ‘biologically meaningful’ [3], as was also the case with another GM corn [29]. The European Food Safety Authority (EFSA) accepted Monsanto's interpretation on NK603 maize [30], like in all other cases. Our study is the first and to date the only attempt to follow up Monsanto's investigation and to determine whether the differences found in the NK603 GM maize-fed rats, especially with respect to liver and kidney function, were not biologically meaningful, as claimed, or whether they developed into serious diseases over an extended period of time. The Monsanto authors adapted Guideline 408 of the Organization for Economic Co-operation and Development (OECD) for their experimental design [3]. Our study design was based on that of the Monsanto investigation in order to make the two experiments comparable, but we extended the period of observation from Monsanto's 90 days to 2 years. We also used three doses of GMOs (instead of Monsanto's two) and Roundup to determine treatment dose response, including any possible non-linear as well as linear effects. This allowed us to follow in detail the potential health effects and their possible origins due to the direct or indirect consequences of the genetic modification itself in the NK603 GM maize, or due to the R herbicide formulation used on the GM maize (and not G alone), or both. Because of recent reviews on GM foods indicating no specific risk of cancer [2,16], but indicating signs of hepatorenal dysfunction within 3 months [1,7], we had no reason to adopt a carcinogenesis protocol using 50 rats per group. However, we prolonged to 2 years the biochemical and hematological measurements and measurements of disease status, as allowed, for example, in OECD protocols 453 (combined chronic toxicity and carcinogenicity) and 452 (chronic toxicity). Both OECD 452 and 453 specify 20 rats per sex per group but require only 50% (ten per sex per group, the same number that we used in total) to be analyzed for biochemical and hematological parameters. Thus, these protocols yield data from the same number of rats as our experiment. This remains the highest number of rats regularly measured in a standard GM diet study, as well as for a full formulated pesticide at very low environmentally relevant levels. We used the Sprague-Dawley strain of rat, as recommended for chronic toxicology tests by the National Toxicology Program in the USA [31], and as used by Monsanto in its 90-day study [3]. This choice is also consistent with the recommendation of the OECD that for a chronic toxicity test, rats of the same strain should be used as in studies on the same substance but of shorter duration [32]. We then also tested for the first time three doses (rather than the two usually employed in 90-day protocols) of the R-tolerant NK603 GM maize alone, the GM maize treated with R, and R alone at very low environmentally relevant doses, starting below the range of levels permitted by regulatory authorities in drinking water and in GM feed. Overall, our study is the first in-depth life-long toxicology study on the full commercial Roundup formulation and NK603 GM maize, with observations on 34 organs and measurement of 56 parameters analyzed at 11 time points for most organs, and utilizing 3 doses. We report here the major toxicological findings on multiple organ systems. As there was no evidence in the literature on GM food safety evaluation to indicate anything to the contrary, this initial investigation was designed as a full chronic toxicity and not a carcinogenicity study. Thus, we monitored in details chronologically all behavioral and anatomical abnormalities including tumors. A full carcinogenicity study, which usually focuses only on observing incidence and type of cancers (not always all tumors), would be a rational follow-up investigation to a chronic toxicity study in which there is a serious suspicion of carcinogenicity. Such indications had not been previously reported for GM foods. Our findings show that the differences in multiple organ functional parameters seen from the consumption of NK603 GM maize for 90 days [3,7] escalated over 2 years into severe organ damage in all types of test diets. This included the lowest dose of R administered (0.1 ppb, 50 ng/L G equivalent) of R formulation administered, which is well below permitted MRLs in both the USA (0.7 mg/L) [33] and European Union (100 ng/L) [34]. Surprisingly, there was also a clear trend in increased tumor incidence, especially mammary tumors in female animals, in a number of the treatment groups. Our data highlight the inadequacy of 90-day feeding studies and the need to conduct long-term (2 years) investigations to evaluate the life-long impact of GM food consumption and exposure to complete pesticide formulations.

Results

Biochemical analyses of the maize feed

Standard biochemical compositional analysis revealed no particular differences between the different maize types and diets, the GM and non-GM maize being classified as substantially equivalent, except for transgene DNA quantification. For example, there was no difference in total isoflavones. In addition, we also assayed for other specific compounds, which are not always requested for establishing substantial equivalence. This analysis revealed a consistent and statistically significant (p < 0.01) decrease in certain phenolic acids in treatment diets, namely ferulic and caffeic acids. Ferulic acid was decreased in both GM maize and GM maize + R diets by 16% to 30% in comparison to the control diet (889 ± 107, 735 ± 89, respectively, vs. control 1,057 ± 127 mg/kg) and caffeic acid in the same groups by 21% to 53% (17.5 ± 2.1, 10.3 ± 1.3 vs. control 22.1 ± 2.6 mg/kg).

Anatomopathological observations and liver parameters

All rats were carefully monitored during the experiment for behavior, appearance, palpable tumors, and infections. At least ten organs per animal were weighed and up to 34 analyzed postmortem, at the macroscopic and/or microscopic levels (Table 1). Due to the large quantity of data collected, it cannot all be shown in one report, but we present here the most important findings. There was no rejection by the animals of the diet with or without GM maize, nor any major difference in body weight (data not shown).

Table 1. Protocol used and comparison to existing assessment and to non-mandatory regulatory tests

The most affected organs in males were the liver, hepatodigestive tract, and kidneys (Table 2; Figure 1A,B,C,D,E,F,G,H,I). Liver abnormalities such as hepatic congestions and macroscopic and microscopic necrotic foci were 2.5 to 5.5 times more frequent in all treatments than in control groups, where only two rats out of ten were affected with one abnormality each. For instance, there were 5 abnormalities in total in the GMO 11% group (2.5 times higher than controls) and 11 in the GMO 22% group (5.5 times greater). In addition, by the end of the experiment, Gamma GT hepatic activity was increased, particularly in the GMO + R groups (up to 5.4 times higher), this probably being reflective of liver dysfunction. Furthermore, cytochrome P450 activity generally increased in the presence of R (either in drinking water or in the GM maize-containing diet) according to the dose and up to 5.7 times greater at the highest dose.

Table 2. Summary of the most frequent anatomical pathologies observed

Figure 1. Anatomopathological observations in rats fed GMO treated or not by Roundup and effects of Roundup alone. Macroscopic (A to D) and microscopic (A' and C') photographs show male left kidneys and livers (E to I) and female pituitaries (J to M), in accordance to Table 2. The number of each animal and its treatment is specified. Macroscopic pale spots (I) and microscopic necrotic foci in liver (G clear-cell focus, H basophilic focus with atypia), and marked or severe chronic progressive nephropathies, are illustrated. In females, pituitary adenomas (K to M) are shown and compared to control (J, rat number and C for control). Apostrophes after letters indicate organs from the same rat.

Transmission electron microscopic observations of liver samples confirmed changes for all treated groups in relation to glycogen dispersion or appearance in lakes, increase of residual bodies and enlargement of cristae in mitochondria (Figure 2, panels 2 to 4). The GM maize-fed groups either with or without R application showed a higher heterochromatin content and decreased nucleolar dense fibrillar components, implying a reduced level of mRNA and rRNA transcription. In the GMO + R group (at the highest dose), the smooth endoplasmic reticulum was drastically increased and nucleoli decreased in size, becoming more compact. In the R alone treatment groups, similar trends were observed, with a partial resumption of nucleolar activity at the highest dose.

Figure 2. Ultrastructure of hepatocytes in male rats from groups presenting the greatest degree of liver pathology. (1) Typical control rat hepatocyte (bar 2 μm except in 4). (2) Effects with Roundup at the lowest dose. Glycogen (G) is dispersed in the cytoplasm. L, lipid droplet; N, nucleus; R, rough endoplasmic reticulum. (3) Details of treatment effects with 22% dietary GMO (bar 1 μm). a, cluster of residual bodies (asterisks); b, mitochondria show many enlarged cristae (arrows). (4) Hepatocytes of animal fed GM maize (GMO) at 22% of total diet. Large lakes of glycogen occur in the cytoplasm. M, mitochondria.

Degenerating kidneys with turgid inflammatory areas demonstrated the increased incidence of marked and severe chronic progressive nephropathies, which were up to two fold higher in the 33% GM maize or lowest dose R treatment groups (Table 2; Figure 1, first line).

Biochemical analyses of blood and urine samples

Biochemical measurements of blood and urine were focused on samples taken at the 15th month time point, as this was the last sampling time when most animals were still alive (in treated groups 90% males, 94% females, and 100% controls). Statistical analysis of results employed OPLS-DA 2-class models built between each treated group per sex and controls. Only models with an explained variance R²(Y) ≥ 80%, and a cross-validated predictive ability Q²(Y) ≥ 60%, were used for selection of the discriminant variables (Figure 3), when their regression coefficients were significant at a 99% confidence level. Thus, in treated females, kidney failures appeared at the biochemical level (82% of the total disrupted parameters). Levels of Na and Cl or urea increased in urine with a concomitant decrease of the same ions in serum, as did the levels of P, K, and Ca. Creatinine and creatinine clearance decreased in urine for all treatment groups in comparison to female controls (Table 3). In GM maize-treated males (with or without R), 87% of discriminant variables were kidney-related, but the disrupted profiles were less obvious because of advanced chronic nephropathies and deaths. In summary, for all treatments and both sexes, 76% of the discriminant variables versus controls were kidney-related.

Figure 3. Orthogonal partial least squares-discriminant analysis (OPLS-DA) for biochemical data (females fed 33% GMO versus controls). (A) First, detailed examples of significant discriminant variables distribution between females fed 33% GMO (bold line) and controls (dotted line). On X axis, animals; on Y axis, serum or urine biochemical values for Na, Cl, estradiol, testosterone. (B) Wider view of OPLS-DA regression coefficients for predictive component, with jack-knifed confidence intervals at 99% confidence level, indicating discriminant parameters versus controls at month 15. U, urinary; UEx, excreted in urine during 24 h; APPT, activated partial thromboplastin time; MCV, mean corpuscular volume; PT, prothrombine time; RBC, red blood cells; ALT, alanine aminotransferase; MCHC, mean corpuscular hemoglobin concentration; A/G, albumin/globulin ratio; WBC, white blood cells; AST, aspartate aminotransferase. Profiles evidence kidney ion leakages and sex hormonal imbalance versus controls.

Table 3. Percentage variation of parameters indicating kidney failures of female animals

Furthermore, in females (Table 3), the androgen/estrogen balance in serum was modified by GM maize and R treatments (at least 95% confidence level, Figure 3). For male animals at the highest R treatment dose, levels of estrogens were more than doubled.

Tumor incidence

Tumors are reported in line with the requirements of OECD chronic toxicity protocols 452 and 453, which require all ‘lesions’ (which by definition include tumors) to be reported. These findings are summarized in Figure 4. The results are presented in the form of real-time cumulative curves (each step corresponds to an additional tumor in the group). Only the growing largest palpable growths (above a diameter of 17.5 mm in females and 20 mm in males) are presented (for example, see Figure 5A,B,C). These were found to be in 95% of cases non-regressive tumors (Figure 5D,E,F,G,H,I,J) and were not infectious nodules. These arose from time to time; then, most often disappeared and were not different from controls after bacterial analyses. The real tumors were recorded independently of their grade, but dependent on their morbidity, since non-cancerous tumors can be more lethal than those of cancerous nature, due to internal hemorrhaging or compression and obstruction of function of vital organs, or toxins or hormone secretions. These tumors progressively increased in size and number, but not proportionally to the treatment dose, over the course of the experiment (Figure 4). As in the case of rates of mortality (Figure 6), this suggests that a threshold in effect was reached at the lower doses. Tumor numbers were rarely equal but almost always more than in controls for all treated groups, often with a two- to threefold increase for both sexes. Tumors began to reach a large size on average 94 days before controls in treated females and up to 600 days earlier in two male groups fed with GM maize (11 and 22% with or without R).

Figure 4. Largest non-regressive tumors in rats fed GMO treated or not by Roundup and effects of Roundup alone. Rats were fed with NK603 GM maize (with or without application of Roundup) at three different doses (11%, 22%, and 33% in their diet; thin, medium, and bold lines, respectively) compared to the substantially equivalent closest isogenic non-GM maize (control, dotted line). Roundup was administered in drinking water at three increasing doses, same symbols, environmental (A), MRL in some agricultural GMOs (B), and half of minimal agricultural levels (C), see ‘Methods’). The largest tumors were palpable during the experiment and numbered from 20 mm in diameter for males and 17.5 mm for females. Above this size, 95% of growths were non-regressive tumors. Summary of all tumors are shown in the bar histograms: black, non-regressive large tumors; white, small internal tumors; grey, metastases.

Figure 5. Examples of female mammary tumors observed. Mammary tumors are evidenced (A, D, H, representative adenocarcinoma, from the same rat in a GMO group) and in Roundup and GMO + Roundup groups, two representative rats (B, C, E, F, I, J fibroadenomas) are compared to controls. A normal representative rat in controls is not shown, only a minority of them having tumors up to 700 days, in contrast with the majority affected in all treated groups. (G) The histological control.

Figure 6. Mortality of rats fed GMO treated or not with Roundup and effects of Roundup alone. The symbols of curves and treatments are explained in the caption of Figure 4. Lifespan during the experiment for the control group is represented by the vertical bar ± SEM (grey area). In bar histograms, the causes of mortality before the grey area are detailed in comparison to the controls (0). In black are the necessary euthanasia because of suffering in accordance with ethical rules (tumors over 25% body weight, more than 25% weight loss, hemorrhagic bleeding, etc.); and in hatched areas, spontaneous mortality.

In female animals, the largest tumors were in total five times more frequent than in males after 2 years, with 93% of these being mammary tumors. Adenomas, fibroadenomas, and carcinomas were deleterious to health due to their very large size (Figure 5A,B,C) rather than the grade of the tumor itself. Large tumor size caused impediments to either breathing or digestion and nutrition because of their thoracic or abdominal location and also resulted in hemorrhaging (Figure 5A,B,C). In addition, one metastatic ovarian cystadenocarcinoma and two skin tumors were identified. Metastases were observed in only two cases; one in a group fed with 11% GM maize and another in the highest dose of R treatment group. Up to 14 months, no animals in the control groups showed any signs of palpable tumors, whilst 10% to 30% of treated females per group developed tumors, with the exception of one group (33% GMO + R). By the beginning of the 24th month, 50% to 80% of female animals had developed tumors in all treatment groups, with up to three tumors per animal, whereas only 30% of controls were affected. A summary of all mammary tumors at the end of the experiment, independent of size, is presented in Table 2. The same trend was observed in the groups receiving R in their drinking water (Figure 4, R treatment panels). The R treatment groups showed the greatest rates of tumor incidence, with 80% of animals affected (with up to three tumors for one female), in each group. Using a non-parametric multiple comparison analysis, mammary tumor incidence was significantly increased at the lowest dose of R compared to controls (p < 0.05, Kruskal-Wallis test with post hoc Dunn's test). All females except one (with metastatic ovarian carcinoma) presented in addition mammary hypertrophies and in some cases hyperplasia with atypia (Table 2). The second most affected organ in females was the pituitary gland, in general around two times more than in controls for most treatments (Table 2; Figure 1J,K,L,M). Again, at this level of examination, adenomas and/or hyperplasias and hypertrophies were noticed. For all R treatment groups, 70% to 80% of animals presented 1.4 to 2.4 times more abnormalities in this organ than controls. The large palpable tumors in males (in kidney and mostly skin) were by the end of the experimental period on average twice as frequent as in controls, in which only one skin fibroma appeared during the 23rd month. At the end of the experiment, internal non-palpable tumors were added, and their sums were lower in males than in females. They were not significantly different from controls, although slightly increased in females (Figure 4, histogram insets).

Mortality

The rates of mortality in the various control and treatment groups are shown as raw data in Figure 6. Control male animals survived on average 624 ± 21 days, whilst females lived for 701 ± 20 days during the experiment, plus in each case, a 5-week starting age at reception of animals and a 3-week housing stabilization period. After mean survival time had elapsed, any deaths that occurred were considered to be largely due to aging. Before this period, 30% control males (three in total) and 20% females (only two) died spontaneously, while up to 50% males and 70% females died in some groups on diets containing the GM maize (Figure 6, panels GMO, GMO + R). However, the rate of mortality was not proportional to the treatment dose, reaching a threshold at the lowest (11%) or intermediate (22%) amounts of GM maize in the equilibrated diet, with or without the R application on the crop. It is noteworthy that the first two male rats that died in both GM maize-treated groups had to be euthanized due to Wilms' kidney tumors that had grown by this time to over 25% of body weight. This was approximately a year before the first control animal died. The first female death occurred in the 22% GM maize feeding group and resulted from a mammary fibroadenoma 246 days before the first control female death. The maximum difference in males was five times more deaths occurring by the 17th month in the group consuming 11% GM maize and in females six times greater mortality by the 21st month on the 22% GM maize diet with and without R. In the female cohorts, there were two to three times more deaths in all treated groups compared with controls by the end of the experiment and deaths occurred earlier in general. Females were more sensitive to the presence of R in drinking water than males, as evidenced by a shorter lifespan (Figure 6, panels R). The general causes of death represented in histogram format within each of the panels in Figure 6, are linked mostly to mammary tumors in females and to problems in other organ systems in males.

Additional file 1. Biochemistry M15 Format: XLS Size: 140KB Download file This file can be viewed with: Microsoft Excel Viewer

Additional file 2. List of blood and urine paramaters with sampling and unit detail Format: PDF Size: 172KB Download file This file can be viewed with: Adobe Acrobat Reader

Additional file 3. Mortality and tumors raw data Format: XLSX Size: 122KB Download file

Additional file 4. Rats identification Format: PDF Size: 90KB Download file This file can be viewed with: Adobe Acrobat Reader

Additional file 5. Raw data legends Format: DOCX Size: 14KB Download file

Discussion

This report describes the first long-term (2-year) rodent (rat) feeding study investigating possible toxic effects arising from consumption of an R-tolerant GM maize (NK603) and a complete commercial formulation of R herbicide. The aims of this investigation were essentially twofold. First, to evaluate whether the signs of toxicity, especially with respect to liver and kidney functions, seen after 90 days' consumption of a diet containing NK603 R-tolerant GM maize [3,7] escalated into serious ill health or dissipated over an extended period of time. Second, to determine if low doses of full commercial R formulation at permitted levels were still toxic, as indicated by our previous in vitro studies [8,9]. The previous toxicity study with NK603 maize employed only this GM crop that had been sprayed with R during cultivation [3]. However, in our study presented here, in addition to extending the treatment period from 90 days to 2 years and in order to better ascertain the source of any ill health observed, we included additional test feeding groups. These consisted of NK603 maize grown without as well as with R application and R alone administered via drinking water. Furthermore, we used three levels of dosing in all cases rather than the two previously used [3], in order to highlight any dose response effects of a given treatment. It is also important to note that our study is the first to conduct blood, urine, and organ analyses from animals treated with the complete agricultural formulation of R and not just G alone, as measured by the manufacturer [35]. Our data show that the signs of liver and kidney toxicity seen at 90 days from the consumption of NK603 GM maize [3,7] do indeed escalate into severe disease over an extended period. Furthermore, similar negative health effects were observed in all treatment groups (NK603 GM maize with or without R application and R alone). What is also evident from our data is that ill effects were not proportional to the dose of either the NK603 GM maize ± R or R alone. This suggests that the observed disease may result from endocrine disruptive effects, which are known to be non-monotonic. Similar degrees of pathological symptoms occurred from the lowest to the highest doses, suggesting a threshold effect [36]. This corresponds to levels likely to arise from consumption or environmental exposure, such as either 11% GM maize in food, or 50 ng/L G equivalent of R-formulation, a level which can be found in some contaminated drinking tap waters and which falls within authorized limits. Death in male rats was mostly due to the development of severe hepatorenal insufficiencies, confirming the first signs of toxicity observed in 90-day feeding trials with NK603 GM maize [7]. In females, kidney ion leakage was evident at a biochemical level at month 15, when severe nephropathies were observed in dead male animals at postmortem, at the anatomopathological level. Early signs of toxicity at month 3 in kidney and liver were also observed for 19 edible GM crops containing pesticide residues [1]. It is known that only elderly male rats are sensitive to chronic progressive nephropathies [37]. Therefore, the disturbed kidney functional parameters may have been induced by the reduced levels of phenolic acids in the GM maize feed used in our study, since caffeic and ferulic acids are beneficial to the kidney as they prevent oxidative stress [38,39]. This possibility is consistent with our previous observation that plant extracts containing ferulic and caffeic acids were able to promote detoxification of human embryonic kidney cells after culture in the presence of R [40]. It is thus possible that NK603 GM maize consumption, with its reduced levels of these compounds, may have provoked the early aging of the kidney physiology, similarly to R exposure causing oxidative stress [41]. Disturbances in global patterns of gene expression leading to disease via epigenetic effects cannot be excluded, since it has been demonstrated that numerous pesticides can cause changes in DNA methylation and histone modification, thereby altering chromatin compaction and thus gene expression profiles [42]. Disturbances that we found to occur in the male liver are characteristic of chronic toxicity, confirmed by alterations in biochemical liver and kidney function parameters. The observation that liver function in female animals was less negatively affected may be due to the known protection from oxidative stress conferred by estrogen [43]. Estrogen can induce expression of genes such as superoxide dismutase and glutathione peroxidase via the MAP kinase-NF-kB signaling pathway, thus providing an antioxidant effect [43]. Furthermore, liver enzymes have been clearly demonstrated as sex-specific in their expression patterns, including in a 90-day rat feeding trial of NK603 GM maize [7]. However, in a long-term study, evidence of early liver aging was observed in female mice fed with R-tolerant GM soy [12]. In the present investigation, deeper analysis at an ultrastructural level revealed evidence of impediments in transcription and other defects in cell nuclear structure that were comparable in both sexes and dose-dependent in hepatocytes in all treatments. This is consistent with the well-documented toxic effect of very low dilutions of R on apoptosis, mitochondrial function, and cell membrane degradation, inducing necrosis of hepatocytes, and in other cell lines [8,9,44,45]. The disruptions of at least the estrogen-related pathways and/or enhancement of oxidative stress by all treatments need further confirmation. This can be addressed through the application of transcriptomic, proteomic, and metabolomic methods to analyze the molecular profile of kidneys and livers, as well as the GM NK603 maize [46-48]. Other possible causes of observed pathogenic effects may be due to disturbed gene expression resulting from the transgene insertional, general mutagenic, or metabolic effects [49,50] as has been shown for MON810 GM maize [51,52]. A consequent disruption of general metabolism in the GMO cannot be excluded, which could lead, for example, to the production of other potentially active compounds such as miRNAs [53] or leukotoxin diols [54]. The lifespan of the control group of animals corresponded to the mean for the strain of rat used (Harlan Sprague-Dawley), but as is frequently the case with most mammals, including humans [55], males on average died before females, except for some female treatment groups. All treatments in both sexes enhanced large tumor incidence by two- to threefold in comparison to our controls and also the number of mammary tumors in comparison to the Harlan Sprague-Dawley strain [56] and overall around threefold in comparison to the largest study with 1,329 Sprague-Dawley female rats [57]. This indicates that the use of historical data to compare our tumor numbers is not relevant, first, since we studied the difference with concurrent controls chronologically (and not only at the end of the experiment, as is the case in historical data), and second, since the diets of historical reference animals may have been contaminated with several non-monitored compounds including GMOs and pesticides at levels used in our treatments. In our study, the tumors also developed considerably faster than in controls, even though the majority of tumors were observed after 18 months. The first large detectable tumors occurred at 4 and 7 months into the study in males and females, respectively, further underlining the inadequacy of the standard 90-day feeding trials for evaluating GM crop and food toxicity [1]. Future studies employing larger cohorts of animals providing appropriate statistical power are required to confirm or refute the clear trend in increased tumor incidence and mortality rates seen with some of the treatments tested in this study. As already stated, our study was not designed as a carcinogenicity study that would have required according to OECD the use of 50 rats per sex per group. However, we wish to emphasize that the need for more rats to provide sufficient statistical power may be biased by the presence of contaminants in the diets used in gathering historical control data, increasing artificially the background of tumors, which would inappropriately be called in this case ‘spontaneous’ or due to the genetic strain. For instance, toxic, hormonal disrupting or carcinogenic levels of pesticides, PCBs, plasticizers, dioxins, or heavy metals may contaminate the diets or drinking water used for the establishment of ‘spontaneous’ tumors in historical data [58-62]. In females, induced euthanasia due to suffering and deaths corresponded mostly to the development of large mammary tumors. This was observed independently of the cancer grade but according to impact on morbidity. These appeared to be related to the various treatments when compared to the control groups. These tumors are generally known to be mostly estrogen-dependent [63]. We observed a strikingly marked induction of mammary tumors in groups administered R alone, even at the very lowest dose (50 ng/L G equivalent dilution in adjuvants). At this concentration in vitro, G alone is known to induce human breast cancer cell growth via estrogen receptors [64]. In addition, R with adjuvants has been shown to disrupt aromatase, which synthesizes estrogen [19], and to interfere with estrogen and androgen receptors in cells [8]. Furthermore, R appears to be a sex endocrine disruptor in vivo in males [10]. Sex steroid levels were also modified in treated rats in our study. These hormone-dependent phenomena are confirmed by enhanced pituitary dysfunction in treated females. An estrogen-modified feedback mechanism may act at this level [65,66]. The similar pathological profiles provoked by the GM maize + R diet may thus be explained at least in part by R residues present in this feed. In this regard, it is noteworthy that the medium dose of the R treatment tested (400 mg/Kg G equivalent) corresponds to acceptable residue levels of this pesticide in some edible GMOs. Interestingly and perhaps surprisingly, in the groups of animals fed with the NK603 GM maize without R application, similar effects with respect to enhanced tumor incidence and mortality rates were observed. For instance, comparing the 11% GMO-treated female group to the controls, the assumption that the tumors are equally distributed is rejected with a level of significance of 0.54% with the Westlake exceedance test [67]. The classical tests of Kolmogorov-Smirnov (one-sided) and Wilcoxon-Mann-Whitney reach α values of significance, which are respectively of 1.40% and 2.62%. A possible explanation for this finding is the production of specific compound(s) in the GM feed that are either directly toxic and/or cause the inhibition of pathways, which in turn generates toxic effects. This is despite the fact that the variety of GM maize used in this study was judged by industry and regulators as being substantially equivalent to the corresponding non-GM closest isogenic line [3,30]. As the total chemical composition of the GM maize has not been measured in detail, the use of substantial equivalence as a concept in risk assessment is insufficient to highlight potential unknown toxins and therefore cannot replace long-term animal feeding trials for GMOs. A cause of the ill effects resulting from NK603 GM maize alone observed in this study could be the fact that it is engineered to overexpress a modified version of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS-CP4) [3], which confers R tolerance. The modified EPSPS is not inhibited by G, in contrast to the wild-type enzyme in the crop. This enzyme is known to drive the first step of aromatic amino acid biosynthesis in the plant shikimate pathway. In addition, estrogenic isoflavones and their glycosides are also products of this pathway [68]. A limited compositional analysis showed that these biochemical pathways were not disturbed in the GM maize used in our study. However, our analysis did reveal that the levels of caffeic and ferulic acids in the GM diet, which are also secondary metabolites of the plant shikimate pathway, but not always measured in regulatory tests, were significantly reduced. This may lower their protective effects against carcinogenesis and mammalian tumor formation [69,70]. Moreover, these phenolic acids, and in particular ferulic acid, may modulate estrogen receptors or the estrogenic pathway in mammalian cells [71]. This does not exclude the possibility of the action of other unknown metabolites. This explanation also corresponds to the fact that the observed effects of NK603 GM maize and R were not additive but reached a threshold. This implies that both the NK603 maize and R may cause hormonal disturbances in the same biochemical and physiological pathways.

Conclusions

In conclusion, the consumption of NK603 GM maize with or without R application or R alone gave similar pathologies in male and female rats fed over a 2-year period. It was previously known that G consumption in water above authorized limits may provoke hepatic and kidney failure [33]. The results of the study presented here clearly indicate that lower levels of complete agricultural G herbicide formulations, at concentrations well below officially set safety limits, can induce severe hormone-dependent mammary, hepatic, and kidney disturbances. Similarly, disruption of biosynthetic pathways that may result from overexpression of the EPSPS transgene in the GM NK603 maize can give rise to comparable pathologies that may be linked to abnormal or unbalanced phenolic acid metabolites or related compounds. Other mutagenic and metabolic effects of the edible GMO cannot be excluded. This will be the subject of future studies, including analyses of transgene, G and other R residue presence in rat tissues. Reproductive and multigenerational studies will also provide novel insight into these problems. This study represents the first detailed documentation of long-term deleterious effects arising from consumption of a GMO, specifically a R-tolerant maize, and of R, the most widely used herbicide worldwide. Taken together, the significant biochemical disturbances and physiological failures documented in this work reveal the pathological effects of these GMO and R treatments in both sexes, with different amplitudes. They also show that the conclusion of the Monsanto authors [3] that the initial indications of organ toxicity found in their 90-day experiment were not ‘biologically meaningful’ is not justifiable. We propose that agricultural edible GMOs and complete pesticide formulations must be evaluated thoroughly in long-term studies to measure their potential toxic effects.

Methods

Ethics

The experimental protocol was conducted in an animal care unit authorized by the French Ministries of Agriculture and Research (Agreement Number A35-288-1). Animal experiments were performed according to ethical guidelines of animal experimentations (CEE 86/609 regulation), including the necessary observations of all tumors, in line with the requirements for a long-term toxicological study [32], up to a size where euthanasia on ethical grounds was necessary. Concerning the cultivation of the maize used in this study, no specific permits were required. This is because the maize was grown (MON-00603-6 commonly named NK603) in Canada, where it is authorized for unconfined release into the environment and for use as a livestock feed by the Canadian Food Inspection Agency (Decision Document 2002-35). We confirm that the cultivation did not involve endangered or protected species. The GM maize was authorized for import and consumption into the European Union (CE 258/97 regulation).

Plants, diets, and chemicals

The varieties of maize used in this study were the DKC 2678 R-tolerant NK603 (Monsanto Corp., USA), and its nearest isogenic non-transgenic control DKC 2675. These two types of maize were grown under similar normal conditions, in the same location, spaced at sufficient distance to avoid cross-contamination. The genetic nature, as well as the purity of the GM seeds and harvested material, was confirmed by qPCR analysis of DNA samples. One field of NK603 was treated with R at 3 L ha⁻¹ (WeatherMAX, 540 g/L of G, EPA Reg. 524-537), and another field of NK603 was not treated with R. Corn cobs were harvested when the moisture content was less than 30% and were dried at a temperature below 30°C. From these three cultivations of maize, laboratory rat chow was made based on the standard diet A04 (Safe, France). The dry rat feed was made to contain 11%, 22%, or 33% of GM maize, cultivated either with or without R, or 33% of the non-transgenic control line. The concentrations of the transgene were confirmed in the three doses of each diet by qPCR. All feed formulations consisted of balanced diets, chemically measured as substantially equivalent except for the transgene, with no contaminating pesticides over standard limits. All secondary metabolites cannot be known and measured in the composition. However, we measured isoflavones and phenolic acids including ferulic acid by standard HPLC-UV. All reagents used were of analytical grade. The herbicide diluted in the drinking water was the commercial formulation of R (GT Plus, 450 g/L of G, approval 2020448, Monsanto, Belgium). Herbicide levels were assessed by G measurements in the different dilutions by mass spectrometry.

Animals and treatments

Virgin albino Sprague-Dawley rats at 5 weeks of age were obtained from Harlan (Gannat, France). All animals were kept in polycarbonate cages (820 cm², Genestil, France) with two animals of the same sex per cage. The litter (Toplit classic, Safe, France) was replaced twice weekly. The animals were maintained at 22 ± 3°C under controlled humidity (45% to 65%) and air purity with a 12 h-light/dark cycle, with free access to food and water. The location of each cage within the experimental room was regularly changed. This 2-year life-long experiment was conducted in a Good Laboratory Practice (GLP) accredited laboratory according to OECD guidelines. After 20 days of acclimatization, 100 male and 100 female animals were randomly assigned on a weight basis into ten equivalent groups. For each sex, one control group had access to plain water and standard diet from the closest isogenic non-transgenic maize control; six groups were fed with 11%, 22%, and 33% of GM NK603 maize either treated or not treated with R. The final three groups were fed with the control diet and had access to water supplemented with respectively 1.1 × 10⁻⁸% of R (0.1 ppb or 50 ng/L of G, the contaminating level of some regular tap waters), 0.09% of R (400 mg/kg G, US MRL of 400 ppm G in some GM feed), and 0.5% of R (2.25 g/L G, half of the minimal agricultural working dilution). This was changed weekly. Twice-weekly monitoring allowed careful observation and palpation of animals, recording of clinical signs, measurement of any tumors, food and water consumption, and individual body weights.

Anatomopathology

Animals were sacrificed during the course of the study only if necessary because of suffering according to ethical rules (such as 25% body weight loss, tumors over 25% body weight, hemorrhagic bleeding, or prostration) and at the end of the study by exsanguination under isoflurane anesthesia. In each case, detailed observations and anatomopathology was performed and the following organs were collected: brain, colon, heart, kidneys, liver, lungs, ovaries, spleen, testes, adrenals, epididymis, prostate, thymus, uterus, aorta, bladder, bone, duodenum, esophagus, eyes, ileum, jejunum, lymph nodes, lymphoreticular system, mammary glands, pancreas, parathyroid glands, Peyer's patches, pituitary, salivary glands, sciatic nerve, skin, spinal cord, stomach, thyroid, and trachea. The first 14 organs (at least ten per animal depending on the sex, Table 1) were weighted, plus any tumors that arose. The first nine were divided into two parts and one half was immediately frozen in liquid nitrogen/carbonic ice. The remaining parts including other organs were rinsed in PBS and stored in 4% formalin before anatomopathological study. These samples were used for further paraffin-embedding, slides, and HES histological staining. For transmission electron microscopy, the kidneys, livers, and tumors were cut into 1 mm³ fragments. Samples were fixed in pre-chilled 2% paraformaldehyde/2.5% glutaraldehyde in 0.1 M PBS pH 7.4 at 4°C for 3 h and processed as previously described [13].

Biochemical analyses

Blood samples were collected from the tail vein of each rat under short isoflurane anesthesia before treatment and after 1, 2, 3, 6, 9, 12, 15, 18, 21, and 24 months: 11 measurements were obtained for each animal alive at 2 years. It was first demonstrated that anesthesia did not impact animal health. Two aliquots of plasma and serum were prepared and stored at −80°C. Then, 31 parameters were assessed (Table 1) according to standard methods including hematology and coagulation parameters, albumin, globulin, total protein concentration, creatinine, urea, calcium, sodium, potassium, chloride, inorganic phosphorus, triglycerides, glucose, total cholesterol, alanine aminotransferase, aspartate aminotransferase, gamma glutamyl-transferase (GT), estradiol, and testosterone. In addition, at months 12 and 24, the C-reactive protein was assayed. Urine samples were collected similarly 11 times, over 24 h in individual metabolic cages, and 16 parameters were quantified including creatinine, phosphorus, potassium, chloride, sodium, calcium, pH, and clearance. Liver samples taken at the end made it possible to perform assays of CYP1A1, 1A2, 3A4, 2C9 activities in S9 fractions, with glutathione S-transferase and gamma-GT.

Statistical analysis

In this study, multivariate analyses were more appropriate than pairwise comparisons between groups because the parameters were very numerous, with samples of ten individuals. Kaplan-Meyer comparisons, for instance, were not used because these are better adapted to epidemiological studies. Differences in the numbers of mammary tumors were studied by a non-parametric multiple comparisons Kruskal-Wallis test, followed by a post hoc Dunn's test with the GraphPad Prism 5 software. Biochemical data were treated by multivariate analysis with the SIMCA-P (V12) software (UMETRICS AB Umea, Sweden). The use of chemometrics tools, for example, principal component analysis (PCA), partial least squares to latent structures (PLS), and orthogonal PLS (OPLS), are robust methods for modeling, analyzing, and interpreting complex chemical and biological data. OPLS is a recent modification of the PLS method. PLS is a regression method used in order to find the relationship between two data tables referred to as X and Y. PLS regression [72] analysis consists in calculating by means of successive iterations, linear combinations of the measured X-variables (predictor variables). These linear combinations of X-variables give PLS components (score vectors t). A PLS component can be thought of as a new variable - a latent variable - reflecting the information in the original X-variables that is of relevance for modeling and predicting the response Y-variable by means of the maximization of the square of covariance (Max cov²(X,Y)). The number of components is determined by cross validation. SIMCA software uses the nonlinear iterative partial least squares algorithm (NIPALS) for the PLS regression. Orthogonal partial least squares discriminant analysis (OPLS-DA) was used in this study [73,74]. The purpose of discriminant analysis is to find a model that separates groups of observations on the basis of their X variables. The X matrix consists of the biochemical data. The Y matrix contains dummy variables which describe the group membership of each observation. Binary variables are used in order to encode a group identity. Discriminant analysis finds a discriminant plan in which the projected observations are well separated according to each group. The objective of OPLS is to divide the systematic variation in the X-block into two model parts, one linearly related to Y (in the case of a discriminant analysis, the group membership), and the other one unrelated (orthogonal) to Y. Components related to Y are called predictive, and those unrelated to Y are called orthogonal. This partitioning of the X data results in improved model transparency and interpretability [75]. Prior to analysis, variables were mean-centered and unit variance scaled.

Competing interests

The author(s) declare that they have no competing interests, and that, in contrast with regulatory assessments for GMOs and pesticides, they are independent from companies developing these products.

Authors’ contributions

GES directed and with JSV designed and coordinated the study. EC, RM, SG, and ND analyzed the data, compiled the literature, and participated in the drafting of the manuscript and final version. MM performed transmission electron microscopy. DH performed OPLS-DA statistical analysis. All authors read and approved the final manuscript.

Acknowledgements

We thank Michael Antoniou for English assistance, editing, and constructive comments on the manuscript. We gratefully acknowledge the Association CERES, for research on food quality, representing more than 50 companies and private donations, the Foundation ‘Charles Leopold Mayer pour le Progrès de l′Homme’, the French Ministry of Research, and CRIIGEN for their major support.

References

1. Seralini G-E, Mesnage R, Clair E, Gress S, de Vendomois J, Cellier D (2011) Genetically modified crops safety assessments: present limits and possible improvements. Environ Sci Eur 23:10 BioMed Central Full Text
2. Domingo JL, Gine Bordonaba J (2011) A literature review on the safety assessment of genetically modified plants. Environ Int 37:734-742 PubMed Abstract | Publisher Full Text
3. Hammond B, Dudek R, Lemen J, Nemeth M (2004) Results of a 13 week safety assurance study with rats fed grain from glyphosate tolerant corn. Food Chem Toxicol 42:1003-1014 PubMed Abstract | Publisher Full Text
4. Hammond B, Lemen J, Dudek R, Ward D, Jiang C, Nemeth M, Burns J (2006) Results of a 90-day safety assurance study with rats fed grain from corn rootworm-protected corn. Food Chem Toxicol 44:147-160 PubMed Abstract | Publisher Full Text
5. Hammond BG, Dudek R, Lemen JK, Nemeth MA (2006) Results of a 90-day safety assurance study with rats fed grain from corn borer-protected corn. Food Chem Toxicol 44:1092-1099 PubMed Abstract | Publisher Full Text
6. Seralini GE, Cellier D, de Vendomois JS (2007) New analysis of a rat feeding study with a genetically modified maize reveals signs of hepatorenal toxicity. Arch Environ Contam Toxicol 52:596-602 PubMed Abstract | Publisher Full Text
7. Spiroux de Vendômois J, Roullier F, Cellier D, Seralini GE (2009) A comparison of the effects of three GM corn varieties on mammalian health. Int J Biol Sci 5:706-726 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
8. Gasnier C, Dumont C, Benachour N, Clair E, Chagnon MC, Seralini GE (2009) Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines. Toxicology 262:184-191 PubMed Abstract | Publisher Full Text
9. Benachour N, Seralini GE (2009) Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chem Res Toxicol 22:97-105 PubMed Abstract | Publisher Full Text
10. Romano MA, Romano RM, Santos LD, Wisniewski P, Campos DA, de Souza PB, Viau P, Bernardi MM, Nunes MT, de Oliveira CA (2012) Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression. Arch Toxicol 86:663-673 PubMed Abstract | Publisher Full Text
11. Krüger M, Schrödl W, Neuhaus J, Shehata A (2013) Field investigations of glyphosate in urine of Danish dairy cows. J Environ Anal Toxicol 3:5
12. Malatesta M, Boraldi F, Annovi G, Baldelli B, Battistelli S, Biggiogera M, Quaglino D (2008) A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing. Histochem Cell Biol 130:967-977 PubMed Abstract | Publisher Full Text
13. Malatesta M, Caporaloni C, Gavaudan S, Rocchi MB, Serafini S, Tiberi C, Gazzanelli G (2002) Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean. Cell Struct Funct 27:173-180 PubMed Abstract | Publisher Full Text
14. Malatesta M, Caporaloni C, Rossi L, Battistelli S, Rocchi MB, Tonucci F, Gazzanelli G (2002) Ultrastructural analysis of pancreatic acinar cells from mice fed on genetically modified soybean. J Anat 201:409-415 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
15. Malatesta M, Perdoni F, Santin G, Battistelli S, Muller S, Biggiogera M (2008) Hepatoma tissue culture (HTC) cells as a model for investigating the effects of low concentrations of herbicide on cell structure and function. Toxicol In Vitro 22:1853-1860 PubMed Abstract | Publisher Full Text
16. Snell C, Bernheim A, Berge JB, Kuntz M, Pascal G, Paris A, Ricroch AE (2012) Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: a literature review. Food Chem Toxicol 50:1134-1148 PubMed Abstract | Publisher Full Text
17. Williams GM, Kroes R, Munro IC (2000) Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans. Regul Toxicol Pharmacol 31:117-165 PubMed Abstract | Publisher Full Text
18. Cox C (2004) Herbicide factsheet - glyphosate. J Pesticide Reform 24:10-15
19. Richard S, Moslemi S, Sipahutar H, Benachour N, Seralini GE (2005) Differential effects of glyphosate and roundup on human placental cells and aromatase. Environ Health Perspect 113:716-720 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
20. Mesnage R, Bernay B, Seralini GE (2013) Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology 313:122-128 PubMed Abstract | Publisher Full Text
21. Adam A, Marzuki A, Abdul Rahman H, Abdul Aziz M (1997) The oral and intratracheal toxicities of ROUNDUP and its components to rats. Vet Hum Toxicol 39:147-151 PubMed Abstract
22. Mesnage R, Defarge N, Spiroux De Vendômois J, Séralini GE (2014) Major pesticides are more toxic to human cells than their declared active principles. Biomed Res Int Vol 2014:Article ID 179691 Publisher Full Text
23. Jobling S, Burn RW, Thorpe K, Williams R, Tyler C (2009) Statistical modeling suggests that antiandrogens in effluents from wastewater treatment works contribute to widespread sexual disruption in fish living in English rivers. Environ Health Perspect 117:797-802 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
24. Krogh KA, Vejrup KV, Mogensen BB, Halling-Sørensen B (2002) Liquid chromatography-mass spectrometry method to determine alcohol ethoxylates and alkylamine ethoxylates in soil interstitial water, ground water and surface water samples. J Chromatogr A 957:45-57 PubMed Abstract | Publisher Full Text
25. Giesy J, Dobson S, Solomon K (2000) Ecotoxicological risk assessment for Roundup® herbicide. In: Ware G (ed) In Reviews of Environmental Contamination and Toxicology, Springer, New York. pp 35-120 Reviews of Environmental Contamination and Toxicology
26. Cox C, Surgan M (2006) Unidentified inert ingredients in pesticides: implications for human and environmental health. Environ Health Perspect 114:1803-1806 PubMed Abstract | PubMed Central Full Text
27. Mesnage R, Clair E, Séralini GE (2010) Roundup in genetically modified plants: regulation and toxicity in mammals. Theorie in der Ökologie 16:31-33
28. Monosson E (2005) Chemical mixtures: considering the evolution of toxicology and chemical assessment. Environ Health Perspect 113:383-390 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
29. Doull J, Gaylor D, Greim HA, Lovell DP, Lynch B, Munro IC (2007) Report of an Expert Panel on the reanalysis by of a 90-day study conducted by Monsanto in support of the safety of a genetically modified corn variety (MON 863). Food Chem Toxicol 45:2073-2085 PubMed Abstract | Publisher Full Text
30. (2003) Opinion of the scientific panel on genetically modified organisms on a request from the commission related to the safety of foods and food ingredients derived from herbicide-tolerant genetically modified maize NK603 for which a request for placing on the market was submitted under Article 4 of the Novel Food Regulation (EC) No 258/97 by Monsanto (QUESTION NO EFSA-Q-2003-002). EFSA J 9:1-14
31. King-Herbert A, Sills R, Bucher J (2010) Commentary: update on animal models for NTP studies. Toxicol Pathol 38:180-181 PubMed Abstract | Publisher Full Text
32. (2009) OECD guideline no. 452 for the testing of chemicals: Chronic toxicity studies: Adopted 7 September 2009. OECD Publishing, Paris, France.
33. [http://waterepagov/drink/contaminants/basicinformation/glyphosatecfm] webcite EPA: Basic information about glyphosate in drinking water. 2014, (last access March).
34. (1998) COUNCIL DIRECTIVE 98/83/EC of 3 November 1998 on the quality of water intended for human consumption. Off J Eur Commun L 330(32):51298
35. German Federal Agency CPFS: Monograph on glyphosate by the German federal agency for consumer protection and food safety.Annex B-5: Toxicol Metabol 1998.
36. Vandenberg LN, Colborn T, Hayes TB, Heindel JJ, Jacobs DR Jr, Lee DH, Shioda T, Soto AM, Vom Saal FS, Welshons WV, Zoeller RT, Myers JP (2012) Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses. Endocr Rev 33:378-455 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
37. Hard GC, Khan KN (2004) A contemporary overview of chronic progressive nephropathy in the laboratory rat, and its significance for human risk assessment. Toxicol Pathol 32:171-180 PubMed Abstract | Publisher Full Text
38. Srinivasan M, Rukkumani R, Ram Sudheer A, Menon VP (2005) Ferulic acid, a natural protector against carbon tetrachloride-induced toxicity. Fundam Clin Pharmacol 19:491-496 PubMed Abstract | Publisher Full Text
39. Sultana S (2011) Attenuation of oxidative stress, inflammation and early markers of tumor promotion by caffeic acid in Fe-NTA exposed kidneys of Wistar rats. Mol Cell Biochem 357:115-124 PubMed Abstract | Publisher Full Text
40. Gasnier C, Laurant C, Decroix-Laporte C, Mesnage R, Clair E, Travert C, Seralini GE (2011) Defined plant extracts can protect human cells against combined xenobiotic effects. J Occup Med Toxicol 6:3 PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text
41. El-Shenawy NS (2009) Oxidative stress responses of rats exposed to Roundup and its active ingredient glyphosate. Environ Toxicol Pharmacol 28:379-385 PubMed Abstract | Publisher Full Text
42. Collotta M, Bertazzi PA, Bollati V (2013) Epigenetics and pesticides. Toxicology 307:35-41 PubMed Abstract | Publisher Full Text
43. Vina J, Borras C, Gambini J, Sastre J, Pallardo FV (2005) Why females live longer than males? Importance of the upregulation of longevity-associated genes by oestrogenic compounds. FEBS Lett 579:2541-2545 PubMed Abstract | Publisher Full Text
44. Benachour N, Sipahutar H, Moslemi S, Gasnier C, Travert C, Seralini GE (2007) Time- and dose-dependent effects of roundup on human embryonic and placental cells. Arch Environ Contam Toxicol 53:126-133 PubMed Abstract | Publisher Full Text
45. Peixoto F (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere 61:1115-1122 PubMed Abstract | Publisher Full Text
46. Jiao Z, Si XX, Li GK, Zhang ZM, Xu XP (2010) Unintended compositional changes in transgenic rice seeds ( Oryza sativa L.) studied by spectral and chromatographic analysis coupled with chemometrics methods. J Agric Food Chem 58:1746-1754 PubMed Abstract | Publisher Full Text
47. Zhou J, Ma C, Xu H, Yuan K, Lu X, Zhu Z, Wu Y, Xu G (2009) Metabolic profiling of transgenic rice with cryIAc and sck genes: an evaluation of unintended effects at metabolic level by using GC-FID and GC-MS. J Chromatogr B Analyt Technol Biomed Life Sci 877:725-732 PubMed Abstract | Publisher Full Text
48. Zolla L, Rinalducci S, Antonioli P, Righetti PG (2008) Proteomics as a complementary tool for identifying unintended side effects occurring in transgenic maize seeds as a result of genetic modifications. J Proteome Res 7:1850-1861 PubMed Abstract | Publisher Full Text
49. Latham JR, Wilson AK, Steinbrecher RA (2006) The mutational consequences of plant transformation. J Biomed Biotechnol 2006:25376 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
50. Wilson AK, Latham JR, Steinbrecher RA (2006) Transformation-induced mutations in transgenic plants: analysis and biosafety implications. Biotechnol Genet Eng Rev 23:209-237 PubMed Abstract | Publisher Full Text
51. Rosati A, Bogani P, Santarlasci A, Buiatti M (2008) Characterisation of 3′ transgene insertion site and derived mRNAs in MON810 YieldGard maize. Plant Mol Biol 67:271-281 PubMed Abstract | Publisher Full Text
52. Abdo E, Barbary O, Shaltout O (2014) Feeding study with Bt corn (MON810: ajeeb YG) on rats: biochemical analysis and liver histopathology. Food Nutri Sci 5:185-195 Publisher Full Text
53. Zhang L, Hou D, Chen X, Li D, Zhu L, Zhang Y, Li J, Bian Z, Liang X, Cai X, Yin Y, Wang C, Zhang T, Zhu D, Zhang D, Xu J, Chen Q, Ba Y, Liu J, Wang Q, Chen J, Wang J, Wang M, Zhang Q, Zhang J, Zen K, Zhang CY (2012) Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Res 22:107-126 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
54. Markaverich BM, Crowley JR, Alejandro MA, Shoulars K, Casajuna N, Mani S, Reyna A, Sharp J (2005) Leukotoxin diols from ground corncob bedding disrupt estrous cyclicity in rats and stimulate MCF-7 breast cancer cell proliferation. Environ Health Perspect 113:1698-1704 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
55. (2012) World health statistics. WHO press, Geneva, Switzerland.
56. Brix AE, Nyska A, Haseman JK, Sells DM, Jokinen MP, Walker NJ (2005) Incidences of selected lesions in control female Harlan Sprague–Dawley rats from two-year studies performed by the National Toxicology Program. Toxicol Pathol 33:477-483 PubMed Abstract | Publisher Full Text
57. Chandra M, Riley MG, Johnson DE (1992) Spontaneous neoplasms in aged Sprague–Dawley rats. Arch Toxicol 66:496-502 PubMed Abstract | Publisher Full Text
58. Hayes TB (2004) There is no denying this: defusing the confusion about atrazine. Biosciences 54:1139-1149 Publisher Full Text
59. Desaulniers D, Leingartner K, Russo J, Perkins G, Chittim BG, Archer MC, Wade M, Yang J (2001) Modulatory effects of neonatal exposure to TCDD, or a mixture of PCBs, p, p'-DDT, and p-p'-DDE, on methylnitrosourea-induced mammary tumor development in the rat. Environ Health Perspect 109:739-747
60. Schecter AJ, Olson J, Papke O (1996) Exposure of laboratory animals to polychlorinated dibenzodioxins and polychlorinated dibenzofurans from commercial rodent chow. Chemosphere 32:501-508 PubMed Abstract | Publisher Full Text
61. Kozul CD, Nomikos AP, Hampton TH, Warnke LA, Gosse JA, Davey JC, Thorpe JE, Jackson BP, Ihnat MA, Hamilton JW (2008) Laboratory diet profoundly alters gene expression and confounds genomic analysis in mouse liver and lung. Chem Biol Interact 173:129-140 PubMed Abstract | Publisher Full Text
62. Howdeshell KL, Peterman PH, Judy BM, Taylor JA, Orazio CE, Ruhlen RL, Vom Saal FS, Welshons WV (2003) Bisphenol A is released from used polycarbonate animal cages into water at room temperature. Environ Health Perspect 111:1180-1187 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
63. Harvell DM, Strecker TE, Tochacek M, Xie B, Pennington KL, McComb RD, Roy SK, Shull JD (2000) Rat strain-specific actions of 17beta-estradiol in the mammary gland: correlation between estrogen-induced lobuloalveolar hyperplasia and susceptibility to estrogen-induced mammary cancers. Proc Natl Acad Sci USA 97:2779-2784 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
64. Thongprakaisang S, Thiantanawat A, Rangkadilok N, Suriyo T, Satayavivad J (2013) Glyphosate induces human breast cancer cells growth via estrogen receptors. Food Chem Toxicol 59C:129-136 Publisher Full Text
65. Popovics P, Rekasi Z, Stewart AJ, Kovacs M (2011) Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats. J Endocrinol 210:71-79 PubMed Abstract | Publisher Full Text
66. Walf AA, Frye CA (2010) Raloxifene and/or estradiol decrease anxiety-like and depressive-like behavior, whereas only estradiol increases carcinogen-induced tumorigenesis and uterine proliferation among ovariectomized rats. Behav Pharmacol 21:231-240 PubMed Abstract | Publisher Full Text | PubMed Central Full Text
67. Deheuvels P (2013) On testing stochastic dominance by exceedance, precedence and other distribution-free tests, with applications. Chapter 10 in Statistical Models and Methods for Reliability and Survival Analysis John Wiley & Sons.
68. Duke SO, Rimando AM, Pace PF, Reddy KN, Smeda RJ (2003) Isoflavone, glyphosate, and aminomethylphosphonic acid levels in seeds of glyphosate-treated, glyphosate-resistant soybean. J Agric Food Chem 51:340-344 PubMed Abstract | Publisher Full Text
69. Kuenzig W, Chau J, Norkus E, Holowaschenko H, Newmark H, Mergens W, Conney AH (1984) Caffeic and ferulic acid as blockers of nitrosamine formation. Carcinogenesis 5:309-313 PubMed Abstract | Publisher Full Text
70. Baskaran N, Manoharan S, Balakrishnan S, Pugalendhi P (2010) Chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis in Sprague–Dawley rats. Eur J Pharmacol 637:22-29 PubMed Abstract | Publisher Full Text
71. Chang CJ, Chiu JH, Tseng LM, Chang CH, Chien TM, Wu CW, Lui WY (2006) Modulation of HER2 expression by ferulic acid on human breast cancer MCF7 cells. Eur J Clin Invest 36:588-596 PubMed Abstract | Publisher Full Text
72. Eriksson L, Johansson E, Kettaneh-Wold N, Wold S (2006) Multi and Megavariate Data Analysis Part I - Principles and Applications. Umetrics AB, Umea, Sweden.
73. Weljie AM, Bondareva A, Zang P, Jirik FR (2011) (1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system. J Biomol NMR 49:185-193 PubMed Abstract | Publisher Full Text
74. Wiklund S, Johansson E, Sjostrom L, Mellerowicz EJ, Edlund U, Shockcor JP, Gottfries J, Moritz T, Trygg J (2008) Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal Chem 80:115-122 PubMed Abstract | Publisher Full Text
75. Eriksson L, Johansson E, Kettaneh-Wold N, Trygg J, Wikström C, Wold S (2006) Multi- and Megavariate Data Analysis Part II. Advanced Applications and Method Extensions. Umetrics, Umea, Sweden.

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